Philippines Health News API

{
    "totalArticles": 111292,
    "articles": [
        {
            "id": "054b567243bb8c2f1389eb6c93fbad4e",
            "title": "Detection of Mycobacterium tuberculosis antigens in urinary extracellular vesicles using real-time immuno-PCR: A non-invasive diagnostic approach for pulmonary tuberculosis",
            "description": "Non-sputum–based diagnostics using readily available clinical samples represent an urgent need in the effort to improve tuberculosis (TB) detection and control. This study evaluated urine as a non-invasive alternative by isolating urinary extracellular vesicles (uEVs), which may facilitate the enrichment of Mycobacterium tuberculosis (Mtb) antigens including MPT-64 and lipoarabinomannan (LAM) being detected in this study. A total of 162 participants were enrolled, including pulmonary TB (PTB) patients (n = 54), latent TB infection (LTBI) subjects (n = 33), other respiratory disease (ORD) controls (n = 58), and healthy controls (n = 17). The uEVs were characterized using nanoparticle tracking analysis, transmission electron microscopy, and western blotting, and subsequently analyzed by real-time immuno-PCR (RT-I-PCR) for simultaneous detection of MPT-64 and LAM. The assay demonstrated ultralow limit of detection (LOD) for purified MPT-64 + LAM (9.7 fg/mL), markedly outperforming indirect ELISA (3.7 ng/mL). Clinically, RT-I-PCR demonstrated 94.44% sensitivity in bacteriologically confirmed PTB, detecting all smear- and/or culture-positive cases and 86.96% of smear- and culture-negative but GeneXpert-positive cases, with an overall specificity of 90.74% among the control groups. In comparison, indirect ELISA identified only 31.48% of bacteriologically confirmed PTB cases while maintaining a high specificity of 96.15%. Receiver operating characteristic (ROC) curve analysis of RT-I-PCR assay indicated high diagnostic accuracy, with area under the curve (AUC) values ranging from 0.9741 to 0.9847. These findings highlight the potential of uEVs-based RT-I-PCR as a highly sensitive, non-invasive approach for PTB diagnosis, while emphasizing the need for further evaluation in larger and more diverse clinical cohorts to develop a scalable, non-invasive diagnostic platform suitable for TB-endemic settings.",
            "content": "Non-sputum–based diagnostics using readily available clinical samples represent an urgent need in the effort to improve tuberculosis (TB) detection and control. This study evaluated urine as a non-invasive alternative by isolating urinary extracellul... [2748 chars]",
            "url": "https://www.nature.com/articles/s41598-026-55896-w?error=cookies_not_supported&code=16a1df45-37df-4897-8080-101aa87ea50d",
            "image": "https://www.nature.com/static/images/favicons/nature/favicon-48x48-b52890008c.png",
            "publishedAt": "2026-06-01T06:15:07Z",
            "lang": "en",
            "source": {
                "id": "7abf0df285fbe93cdccffcc7c4088737",
                "name": "Nature",
                "url": "https://www.nature.com"
            }
        },
        {
            "id": "32dadc3498fa9c86a039958e86f54209",
            "title": "No need to delay: 5 places to go for preventive health care today",
            "description": "No need to delay: 5 places to go for preventive health care today",
            "content": "For many adults today, health is no longer a matter of treating illness; it is about staying well so life does not slow down. Beyond the physical and financial burden of getting sick, unplanned disruptions to work and personal responsibilities have p... [3045 chars]",
            "url": "https://www.manilatimes.net/2026/06/01/news/no-need-to-delay-5-places-to-go-for-preventive-health-care-today/2355341",
            "image": "https://cdnx.premiumread.com/?url=https://www.manilatimes.net/theme_manilatimes/images/TMT_1920x1008.jpg&w=1920&q=100&f=webp&v=1",
            "publishedAt": "2026-06-01T03:58:00Z",
            "lang": "en",
            "source": {
                "id": "e3ad8bbf7dbe3c3defc56a8393455066",
                "name": "The Manila Times",
                "url": "https://www.manilatimes.net"
            }
        },
        {
            "id": "516c7ed430283912a72008b1e65fc31e",
            "title": "Largest On Record As Suspected Cases Pass 1,000",
            "description": "A rare and deadly strain of the Ebola virus is spreading rapidly through a region of the Democratic Republic of the Congo (DRC) that has never previously",
            "content": "A rare and deadly strain of the Ebola virus is spreading rapidly through a region of the Democratic Republic of the Congo (DRC) that has never previously experienced the disease, pushing suspected cases past 1,000 this week amid severe logistical hur... [8473 chars]",
            "url": "https://healthpolicy-watch.news/congo-ebola-outbreak-now-third-largest-on-record-as-suspected-cases-pass-1000/",
            "image": "https://i0.wp.com/healthpolicy-watch.news/wp-content/uploads/2026/05/MSF249714.jpg",
            "publishedAt": "2026-05-31T23:38:31Z",
            "lang": "en",
            "source": {
                "id": "d534448f93dee061e92d57c0d121e82f",
                "name": "Health Policy Watch",
                "url": "https://healthpolicy-watch.news"
            }
        }
    ]
}